Part:BBa_K5292306:Design

PelB

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 54
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

In this project, we did not specifically design the signal peptide. Instead, we selected signal peptides with potential secretion capabilities from an existing signal peptide library based on model predictions for experimental validation. The selection and use of the signal peptide aimed to explore its effectiveness in guiding the secretion of target proteins and provide data support for future optimization.

Source

This signal peptide originates from library of signal peptides in Escherichia coli,and was used to express Pectate lyase B.